Vitamin c determination by iodine titration lab report. Lab 4 2022-12-15
Vitamin c determination by iodine titration lab report
Vitamin C, also known as ascorbic acid, is a water-soluble vitamin that is essential for the proper functioning of the human body. It plays a key role in the synthesis of collagen, the main structural protein in the body, as well as in the synthesis of neurotransmitters and hormones. Vitamin C is also a powerful antioxidant, meaning it helps to protect cells from damage caused by free radicals.
The determination of vitamin C content in a sample can be done through a variety of methods, including colorimetry and spectrophotometry. However, one of the most commonly used methods is iodine titration. This method is based on the reaction between vitamin C and iodine, which results in the formation of a blue-black complex known as iodopurpurin.
In the iodine titration lab, a known volume of the sample containing vitamin C is placed in a flask. An iodine solution is then added to the flask, and the volume of the iodine solution required to react with all of the vitamin C in the sample is determined through titration. The endpoint of the titration is the point at which the iodine solution has completely reacted with the vitamin C in the sample, resulting in the formation of the iodopurpurin complex.
To determine the vitamin C content of the sample, a standard curve is prepared by titrating known concentrations of vitamin C with the iodine solution. The concentration of vitamin C in the sample can then be determined by comparing the volume of iodine solution required to react with the sample to the standard curve.
There are several advantages to using the iodine titration method for the determination of vitamin C. One of the main advantages is that it is a relatively simple and quick method that does not require specialized equipment. Additionally, the method is highly accurate and reproducible, making it a reliable method for the determination of vitamin C.
There are, however, some limitations to the iodine titration method. One limitation is that the method is not suitable for the determination of vitamin C in the presence of other reducing agents, as these agents can interfere with the reaction between vitamin C and iodine. Additionally, the method is not sensitive to low concentrations of vitamin C, making it less suitable for the analysis of samples with low vitamin C content.
Overall, the iodine titration method is a reliable and widely used method for the determination of vitamin C in a variety of samples. While it does have some limitations, it remains an important tool for the analysis of vitamin C content in a range of different samples.
Determination of Vitamin C using Iodometric titration
Based on this value, the volume of the fruit juice samples, the volume of the whole fruits and the concentration of ascorbic acid in our fruit juice samples I calculated the number of fruits we would have to take to cover the 75g need, as well as the overall weight and volume the fruits we would have to take would take. Determine how much titrant was required for your standard. With that I mean that oranges can be easily pealed and eaten as snack what means it would be easy to use them as vitamin c source. If we would just consider the number of boxes we would have to take to store the needed amount of vitamin c for the journey taking the fruit juice would be the better option since fewer boxes would be needed. Freeman: San Francisco, 1976, pp.
Vitamin C Titration Lab Report
When investigating the fruits we did not focus on the varying sizes of fruits, as well as on the thickness of their peel or their eatability. The standardization of the iodine solution using ascorbic acid was accurate and precise however there was a calculation error that is exhibited in this report. A few of the side impacts are the Macromolecules Lab Report 1486 Words 6 Pages The iodine test determines the presence of starch in biological materials. This task was performed by titration of vitamin C in given fruits followed by standardization of iodine solution. The end point of the titration can be understood by the color change during titration. Repeat the titration until you have at least three measurement that agree to within 0. A 0 M KIO 3 solution is prepared by accurately weighing approximately 1g of solid reagent and dissolving it in the 500-mL volumetric flask.
Lab Report Vit C Titration new
In this experiment, the addition of iodine to vitamin C in acidic solution with the presence of starch was stopped once color of solution started to change dark purple color from colorless …show more content… This experiment was done by using a mandarin orange. Next we add 20mL of deionized water and anthocyanin indicator drop Column Chromatography Lab Report 1696 Words 7 Pages Experiment 7: Column Chromatography of Food Dye Arianne Jan D. Hypothesis The process of titration will result in a color change that will indicate the solution reaching. Determination of Vitamin C in a Sample The procedure for determination vitamin C in a sample is identical to that for standardizing the DCIP solution. Standardization of the iodine solution with the vitamin C standard solution and sample solution.
The amount of vitamin C in a sample will be determined by redox titration using the reaction shown in Scheme 1 between ascorbic acid and 2, 6-dichloroindophenol DCIP. In order to be able to determine the concentration of 1 in the fruit samples we needed to prepare a standardized iodine solution. Record the initial and final volume readings on the buret in laboratory notebook data table. The unknown number of the solution was 152. Howevever, when the all the vitamin C is oxidized, iodine and triiodide will be present, which react with starch to form a blue-black complex.
Vitamin C Determination by Iodine typemoon.org
Standardization of the 2, 6-Dichloroindophenol Solution Before using DCIP to quantitatively measure vitamin C, you must know the concentration of the DCIP solution. In this lab, the student also learns the technique of using titration. You and your laboratory partner will need to develop this hypothesis and submit a one-page typed proposal, which must be approved by your instructor before you can begin work. Standardization of the Iodine Solution. Collect your values, and those of other groups that worked with you, in a single table in your laboratory notebook. The redox reaction is better than an acid-base titration since there are additional acids in a juice, but few of them interfere with the oxidation of ascorbic acid by iodine.
Vitamin C Determination By Iodine [k546v281gwn8]
Vitamin C, the Common Cold, and the Flu; W. Calculate the ml of titrant used for each flask. Please remember that certain activities are not easily performed in the laboratory i. However, it isdifficult to detect endpoints using iodine colouration alone therefore a starch is added, which formed an intensely blue coloured complex with iodine. As 49 ml of iodine is required for the color change containing 20 ml ascorbic acid solution, the dilution was done 5 times to that of the solution.
Vitamin C Determination by Iodine Titration
Therefore, you may need to perform some sample preparations, such as cooking, before coming to the laboratory. The number of fruits we would have to take would be slightly different. Dilute to 250 ml with distilled water in a volumetric flask. Besides plays a paramount role as an antioxidant and free radical scavenger, it has been suggested to be an effective antiviral agent 2. The only restriction on your choice of topic is that your work may not involve vertebrate animals or any samples of human origin.
So as we see even if we would have to store a slightly smaller number of boxes on the ship if we would take the fruit juice, taking the whole fruits would be the favored option. Titrate until the endpoint is reached. Take the measurements you obtained and average them. Include in your proposal a list of the samples that you will analyze you will supply the samples and outline any sample preparation that is not described in the experimental section of this exercise. Some possible topics are given below, but you are not limited to this list creativity will be rewarded. Reaction of Ascorbic Acid or Vitamin C 1 and Iodine 2 in which 1 is oxidized and 2 is reduced eq 1.
Determination of Vitamin C
Calculate the uncertainty in the amount of vitamin C in your sample at the 95% confidence limit. Ascorbic acid can donate a hydrogen atom to a free radical, and thus stop these reactions from occuring. Class data and calculated data based on Juice Titrations Lime Lemon Orange Volume of juice titrated mL Average 50mL Class average 20mL 20. Vitamin C Analysis Authors: B. Label the flask as your vitamin C standard solution.