Unknown lab report. Identification of Unknown Bacteria Lab typemoon.org 2022-12-31

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A lab report is a document that describes the results and findings of a laboratory experiment or investigation. It is typically written for a scientific audience and can be found in academic journals, research reports, and other scientific literature.

The purpose of a lab report is to communicate the results of a scientific experiment or investigation in a clear, concise, and organized manner. It should provide a detailed and objective description of the methods and procedures used, the results obtained, and the conclusions drawn from the data.

The structure of a lab report typically includes the following sections:

A lab report is an important tool for communicating scientific research and findings, and it is essential that it is written in a clear and concise manner. It should be carefully proofread and edited to ensure that it is free of errors and accurately represents the work that was conducted.

Unknown Lab typemoon.org

unknown lab report

The biochemical tests were chosen from the unknown identification tables that were in the lab manual. Living bacteria were placed on a paper towel and saturated with a chromogenic reducing agent. This could have been due to the fact that too much of the unknown organism was fixed to the slide before gram staining. For example, if your unknown sample was Staphylococcus aureus, you might look for articles that describe the impact of S. The casein test on the milk agar also came back positive which lead to the identity of alternate 8 being B. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. The unknown bacteria is Staphylococcus aureus.

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Unknown Organism Lab typemoon.org

unknown lab report

An inoculating loop was sterilized, and the urea test tube was inoculated with the bacteria. This MSA plate was incubated at 37 degrees Celsius for 48 hours. After incubating the TSA plate for 24-48 hours the bacterium grew and their characteristics were recorded. MediLexicon International, 8 June 2011. They could develop HUS hemolytic uremic syndrome , a type of kidney failure, which can be potentially fatal 2.

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Unknown Bacteria Lab Report

unknown lab report

The Staphylococcus aureus and Enterococcus faecalis are positive to mannitol. It builds resistance against a lot of antibiotics and even chemotherapeutic agents. Afterwards, the bacterium was determined to be positive for lysine decarboxylation and citrate. The oxidase test was performed only on gram - bacteria and was used to test for the presence of cytochrome oxidase. It is most dangerous to cystic fibrosis patients and is involved and complicates 90% of cystic fibrosis deaths.

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Example of Unknown Lab Report, Microbiology

unknown lab report

There was growth on the NA plate and there was 2 distinct color which maybe a manifestation of 2 or more different types of bacteria. After performing these tests and narrowing down the unknown organisms list given in class it seems as if the unknown organisms is Klebsiella pneumoniae. The other tests, the gelatin hydrolysis used for a confirmatory between B. The Iodine was used as mordant and become colorless when alcohol was used. In order for an individual to receive treatment for a certain bacterium, this bacterium must first be identified. Escherichia coli, also known as E.

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(Sample) Unknown Lab Report

unknown lab report

Ethanol is added to get rid of the outer membrane of Gram-negative bacteria which makes them colorless at this point then safranin is added as a counterstain which makes the Gram-negative bacteria pink and the Gram-positive bacteria stay purple. The quadrant streak helped spread out the bacteria and made it easier for the two different bacteria to make colonies that could be differentiated by the naked eye. After the VP test was done, an Indole Test was performed and incubated until Tuesday. The EMB test produced green metallic colonies, which was a big flag that the bacterium might be Escherichia coli. In fact, the article will sound a bit similar to your report in that it will talk about objectives, materials and methods, and will have a conclusion and a discussion. The catalase-positive bacteria include strict aerobes. In addition, students will receive their AHA Cards the day of class! Flowchart 2 is also available for the results of this unknown.

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Unknown Sample Lab Report

unknown lab report

The flow charts show how the conclusions were reached. The Methyl Red test was positive for the presence of glucose fermentation. After being extra careful a second isolation was performed, and when the Gram stain was observed there were only Gram-positive rods. Tables 1 and 2 lists the tests, purposes, reagents used and the results of each test. The Gram negative Bacillus rods were simpler to identify as they only had two options.

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Unknown Lab Report

unknown lab report

RESULTS: For the Gram negative rods, the indole test came back positive which left me between Escherichia coli or Proteus vulgaris. Returning to the original unknown stock 123, a quadrant streak plate was done with a sterilized inoculating look on a mannitol salt agar, which inhibits the growth of gram negative bacteria. On November 5, more tests were performed to narrow down the list given in class of the unknown organisms. For more information, please visit these links: For this assignment you will cite your sources using the Please note this style is particular to this one journal; to see some examples of formatted citations check the class handout linked in this section. Please note that due to formatting issues the flow charts had to be removed. . Only one type of colony had grown on my MAC plate indicating a gram negative species, which I chose to be organism A.

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Unknown Lab Report

unknown lab report

The Urea test was implemented to name the unknown. The only problem that was encountered appeared when trying to isolate the gram positive bacteria. In the health field it is important for physicians to understand this so the patient can be accurately treated. The only gram negative bacteria that shows a positive result on the Casein Test is Pseudomonas aeruginosa, therefore correctly confirming the unknown gram negative bacteria. The bacteria was tested on the mannitol test and turns read mannitol to yellow. After incubation, this nutrient agar had great results with many isolated colonies. Ciobanu 2 Identification of Unknown Bacteria Lab Report Objective: The main objective of this lab is to determine the genus and species of an unknown bacteria.

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How to Write a Microbiology Unknown Lab Report

unknown lab report

The selection process was started with a methyl red test which led to the conclusion of B. This lab demonstrates an understanding of utilizing various experiments to identify the species and family of unknown bacteria. In order to do the streak method, an inoculating loop was sterilized with a Bunsen burner and put into the unknown specimen. First, a drop of culture was aseptically transferred to a slide and dried. This test was done in accordance with the procedure that is described on page 200 in the lab manual.

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