A microhematocrit is a laboratory test used to measure the volume of red blood cells in a sample of blood. It is commonly used to diagnose anemia, which is a condition characterized by a low red blood cell count. The test is performed by spinning a small sample of blood in a special tube called a hematocrit tube, which separates the red blood cells from the other components of the blood, such as plasma and white blood cells.
The test is performed by taking a small sample of blood from a patient, usually by drawing it from a vein in the arm. The sample is then placed in a hematocrit tube, which is a thin, vertical tube with a graduated scale on the side. The tube is then placed in a centrifuge, a machine that spins the tube at high speeds to separate the blood cells based on their weight.
The red blood cells are denser than the other components of the blood, so they are pushed to the bottom of the tube by the centrifuge. The volume of the red blood cells is then measured by reading the scale on the side of the tube. This measurement is expressed as a percentage, known as the hematocrit value.
The hematocrit value is used to diagnose anemia, as well as other conditions related to the production, destruction, or loss of red blood cells. For example, a low hematocrit value may indicate anemia, while a high hematocrit value may indicate dehydration or a condition called polycythemia, in which the body produces too many red blood cells.
The microhematocrit test is a simple and reliable way to measure the volume of red blood cells in the blood. It is commonly used in conjunction with other tests, such as a complete blood count, to diagnose and monitor a variety of medical conditions.
What is the purpose of the Microhematocrit test?
It is important that the tubes are sealed thoroughly. As the distribution shifts to the right or toward higher values with age, as it does for women until ages 55-64 years, the mean increases. Medical Definition of microhematocrit 1 : a procedure for determining the ratio of the volume of packed red blood cells to the volume of whole blood by centrifuging a minute quantity of blood in a capillary tube coated with heparin. . Allow blood to track up the tube. Procedure for determining packed cell volume by the hematocrit method. PRINCIPLE: When anticoagulated whole blood is centrifuged, the space occupied by the packed red blood cells is defined as the hematocrit HCT , and is expressed as the percent of red blood cells in a volume of whole blood.
What is the principle of Microhematocrit determination? [Fact Checked!]
Hematocrit PCV is the measure of the ratio of the volume occupied by the red blood cells to the volume of whole blood. How does hemolysis affect the microhematocrit? Having too few or too many red blood cells can be a sign of certain diseases. A decreased hematocrit value can indicate a condition such as anemia or the presence of bleeding in a patient. Common symptoms include fatigue, weakness, and low energy. The microhematocrit only requires a small amount of blood and it is a CLIA-waived test. Conductivity on blood gas analyzers In POCT, blood gas analyzers measuring multiple STAT parameters are often used. Is Microhematocrit a CLIA waived test? Some methods are not suitable in certain applications due to e.
For the microhematocrit method, after filling the blood in a capillary tube, the two ends of the tube commonly 75 mm long, 1 mm diameter are sealed with clay sealant or heat. For men, a normal range is 40. Understanding Your Lab Test Results. In healthy adult individuals the red blood cells constitute approx. A hematocrit of 0.
A hematocrit level below the normal range, meaning the person has too few red blood cells, is called anemia. What precautions should be observed when performing a Microhematocrit? Why is heparin used in microhematocrit tubes? What is the Microhematocrit? Different hemoglobin measuring methods and their applications will be discussed in another article. Analytical artifacts in hematocrit measurements by whole-blood chemistry analyzers. What does it mean if hematocrit is high? The reference method recommended by NCCLS of determining hematocrit or packed cell volume PCV is centrifugation. Normal level for women is 36% to 48%.
Then the blood-filled end is sealed with clay, and placed in a slot in the hematocrit centrifuge. A distinct column of packed erythrocytes is visible in one end of the capillary tube Fig. What is the purpose of the Microhematocrit test? Some blood gas analyzers offer this possibility, either by measuring hematocrit by conductivity or by calculating hematocrit from ctHb. This separates the blood into layers. What safety precautions should be observed when performing a microhematocrit? How do you fill a Microhematocrit tube? Normal level for women is 36% to 48%. Name a condition that could cause a decreased hematocrit value.
The blood sample is drawn into a capillary and centrifugated, and then the ratio can be measured and expressed as a decimal or percentage fraction. Sometimes, higher or lower hematocrit levels are signs of serious medical conditions. It provides hematocrit values HCT for testing in biochemistry, immunity, genetics, blood separation and for general clinical tests. A Test Details How do healthcare providers obtain blood for hematocrit levels? Clin Chem 1995; 41, 2: 306-11. Microhematocrit centrifuges are intended to aid in the diagnosis of blood disorders and diseases. How do hematocrit tubes work? Biochemical Basis of Pediatric Disease. A hematocrit performed on blood in a capillary tube.
Red blood cells are important because they carry oxygen throughout your body. What factors affect hematocrit levels? Packed cell volume, PCV, n — The measure of the ratio of the volume occupied by the red blood cells to the volume of whole blood, expressed as a fraction. The hematocrit of infants reaches the level of adult hematocrit by approx. Observe Standard precautions; use plastic or Mylar-coated self-sealing tubes; close centrifuge lids securely; never open centrifuge until rotor has stopped; dispose of all sharps in appropriate containers. What is the value of a microhematocrit tube? When do healthcare providers order hematocrit tests? Capillary tubes are filled by capillary forces.