Gram staining observation result and conclusion. Gram Stain: What It Is, Purpose, Procedure & Results 2022-12-11

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Korean Airlines Flight 801 was a scheduled flight from Seoul, South Korea to Guam that crashed on August 6, 1997, killing 228 of the 254 people on board. The crash of Flight 801 was a tragic and devastating event that had a lasting impact on the families of the victims, as well as on the aviation industry as a whole.

The flight was operated by a Boeing 747-300 aircraft and was piloted by Captain Park Chang-Kyung, a veteran pilot with over 13,000 hours of flying experience. The plane took off from Seoul's Gimpo International Airport at 10:58 p.m. local time and was scheduled to arrive in Guam at 4:01 a.m. the following morning.

However, just minutes after takeoff, the plane encountered heavy rain and strong winds. As the plane flew over the Pacific Ocean, it encountered severe turbulence and the pilots struggled to maintain control. Despite their efforts, the plane crashed into a hillside near Nimitz Hill, just a few miles from the airport in Guam.

The cause of the crash was later determined to be pilot error. The pilots had failed to properly execute the approach to the airport and had not followed the proper procedures for landing in poor weather conditions. In addition, the pilots were found to have inadequate training in the use of the plane's instrument landing system (ILS), which is a critical tool for navigating and landing in poor weather conditions.

The crash of Korean Airlines Flight 801 was a tragic reminder of the importance of proper training and safety procedures in the aviation industry. It also highlighted the need for improved communication and coordination between pilots and air traffic controllers in order to prevent similar tragedies from occurring in the future.

In the wake of the crash, Korean Airlines implemented a number of safety improvements, including increased training for pilots, improved communication and coordination between pilots and air traffic controllers, and the adoption of new technology to help navigate and land planes in poor weather conditions.

The families of the victims of the crash were also left to deal with the aftermath of the tragedy. Many of them filed lawsuits against Korean Airlines, seeking compensation for their losses. In the end, the families of the victims received a settlement from the airline, though it could never fully compensate for the loss of their loved ones.

The crash of Korean Airlines Flight 801 will always be remembered as a tragic and devastating event. It serves as a reminder of the importance of safety and proper training in the aviation industry and the need to continually strive for improvement in order to prevent similar tragedies from occurring in the future.

Gram Staining, its Principle, Procedure, and Results

gram staining observation result and conclusion

This methos will be great to bacteria that have cell wall but this method cannot cell acid fast bacteria which is bacteria that do not have cell wall. What are the risks of a Gram stain test? Gram-positive and gram-negative cell wall structure. What will be the colors of Gram positive and Gram negative bacteria at the conclusion of the technique? Whereas, in the Gram-Positive cell wall the decolorizer dehydrates the peptidoglycan layer and traps the CVI complex within the cell. To be visible on a slide, organisms that stain by the Gram method must be present in about 10 4 to 10 5 organisms per milliliter of centrifuged fluid. Collecting the Gram stain sample A Gram stain test involves your healthcare provider collecting a sample from a site of suspected infection. Decolorization ethanol Add drops of ethanol one at a timeuntil the runoff is clear. Gram staining is initially established by the physician Hans Christian Gram, which was from Denmark.

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Conclusion In conclusion Gram staining method is widely use to differentiate

gram staining observation result and conclusion

After decolorization, the gram-positive cell remains purple. Save them for 24 hours or 1 dayand filter. Staining Results and Conclusion I. Positive Gram stain If your test result reveals a positive Gram stain, it means that bacteria were present in your sample. Both gram positive and gram negative bacteria have molecules called peptidoglycans in their cell walls. It has also antigenic properties that stimulate specific immune responses when it is released from the cell wall after cell death. It was first described by Danish pathologist Christian Gram in 1884.

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What is the conclusion of the gram staining technique?

gram staining observation result and conclusion

This staining technique uses two stains; crystal violet as primary stain and safranine as a counterstain. Gram-negative organisms may not decolorize properly. It is also useful for simple differentiation of bacteria into gram-positive and gram-negative groups. Some of them have improved the method, while others include some minor technical variants of no value. Rinse the slide with water.

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Lab 5

gram staining observation result and conclusion

It is described as the corner stone of clinical laboratory. Who performs a Gram stain test? Broadway Edmond Oklahoma 73034. Proper handling of visual aids 5 2. More understanding the types and morphology of bacteria. Smear Preparation: The 1 st thought is the precise training of smear.

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Comparision of original gram stain and its modification in the gingival plaque samples

gram staining observation result and conclusion

A Gram stain helps diagnose harmful bacteria. Retrieved December 26, 2017 from ii. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. Make your observations using oil immersion 1000X. This wall forms in a mesh like formation of three layers of alternating material.

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Gram Stain: Introduction, Principle, Procedure, Result and Interpretation

gram staining observation result and conclusion

Retrieved October 9, 2017 from iii. Using a drawing form draw the organisms Premium Staining Gram staining Bacteria Stain Exercise 3. When fluid samples for example urine or CSF is receive it look to disappear after the staining process, a wax spot, positioned near the smeared part on the Same sideon the glass slide after the staining process to escape from wax artifacts will decrease prevention in finding the sample below the microscope. Bacteria having cell walls with a thick layer of peptidoglycan will resist decolorization of primary stain and appear violet or purple. Many of the stains they use color the bacteria cells directly and are called direct stains.

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Gram Staining: Principle, Procedure, Results • Microbe Online

gram staining observation result and conclusion

Step Procedure Outcome Primary stain crystal violet Add several drops of crystal violet to the smear and allow it to sit for 1 minute. You may need further testing to identify the specific type. Two steps in the Gram stain serve to 'trap' crystal violet within the peptidoglycan. Gram stain reaction and cellular features of the culture. All the smears were heat fixed and stain by the following procedures. They consist of two domains: Archaea and Bacteria.

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Staining Results and Conclusions. typemoon.org

gram staining observation result and conclusion

Biological stains are used for the medical and biological industries to aid in detection of structures within tissues. Gram Stain Mechanism: Gram Positive Cell Wall: Gram-positive bacteria have a thick mesh-like cell wall which is made up of peptidoglycan 50-90% of cell wall , which stains purple. The principle of gram stain is that some organisms are not decolorized and retain color of basic stain; example gentian violet gram positive organisms while the others lose all gentian violet and take up the counter stain; example dilute carbol fuschion or safranin gram negative organisms. Gram-negative organisms Gram-negative bacteria have cell walls with thin layers of peptidoglycan 10% of the cell wall and high lipid fatty acid content. While some types of bacteria are harmless or even beneficial, others can cause infections and disease.

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