Fluorescence spectroscopy lab report quinine. Quinine fluorescence spectra. A dry 2022-12-11

Fluorescence spectroscopy lab report quinine Rating: 6,1/10 130 reviews

Fluorescence spectroscopy is a powerful analytical technique that utilizes the phenomenon of fluorescence to identify and quantify molecules in a sample. In a fluorescence spectroscopy lab report, the focus is typically on the analysis of a specific compound or group of compounds using this technique. In this case, the compound of interest is quinine, which is a naturally occurring alkaloid that is commonly used as a medicinal agent to treat malaria.

The first step in a fluorescence spectroscopy lab report on quinine is to prepare the sample for analysis. This typically involves dissolving the quinine in a suitable solvent, such as water or methanol, and then filtering the solution to remove any impurities. The concentration of the quinine solution can then be determined using a suitable reference standard, such as a known concentration of a similar compound.

Next, the sample is irradiated with light of a specific wavelength that is absorbed by the quinine molecules. This causes the quinine molecules to become excited and emit light of a longer wavelength, which is known as fluorescence. The intensity of the emitted light is then measured as a function of the wavelength, which is known as the fluorescence spectrum.

The fluorescence spectrum of quinine is typically characterized by a strong peak at a wavelength of around 350 nm, which is known as the excitation maximum. The intensity of the emitted light at this wavelength is directly proportional to the concentration of quinine in the sample. Therefore, by comparing the intensity of the emitted light to a reference standard, it is possible to determine the concentration of quinine in the sample.

In addition to the excitation maximum, the fluorescence spectrum of quinine may also exhibit other peaks at longer wavelengths. These peaks are known as emission maxima and are typically caused by the presence of impurities or other substances in the sample that interfere with the fluorescence of quinine.

To conclude, fluorescence spectroscopy is a powerful analytical technique that can be used to identify and quantify quinine in a sample. By measuring the intensity of the emitted light as a function of wavelength, it is possible to determine the concentration of quinine and identify any impurities or interfering substances in the sample. This information is critical for the development and quality control of medicinal agents containing quinine, as well as for the study of the biological effects of this compound.

fluorescence lab report

fluorescence spectroscopy lab report quinine

The peak area is generated by integrating peak function from the lower bound to the upper bound of the peak. . Quantify Quinine in Beverages Using the Agilent Cary Eclipse Spectrofluorometer and a Fiber Optic Dip Probe. Spectroscopy is the analysis of spectra, typically light or mass spectra, where the spectrum of a source is used to determine the composition of a substance. Learn More The nature of such luminescence can be due to various intramolecular mechanisms, one of which is the transition of an excited molecule from the lowest singlet vibrational level to the ground state. Aniline is an example.

Next

Quinine fluorescence spectra. A dry

fluorescence spectroscopy lab report quinine

To analyze our data, we used a fluorometer with a quartz cuvette under optically thin conditions. Last, in the tube labeled five we had a solution of 4. Quenching is a defined as anything that lowers the intensity. UV-Vis spectrometer is zeroed but filling cuvette with de ionised water and zeroing the instrumentation. For example, the light omitted by fireflies, deep sea fish and glow sticks is a result of this process. Quinine is also a good example of a molecule which can undergo fluorescent quenching by the presence of other elements e.

Next

Spectroscopy Laboratory Report

fluorescence spectroscopy lab report quinine

In certain cases, electrons cross over to the triplet state T 1 via intersystem crossing. Then it becomes evident that there is a linear dependence with the coefficient K between the concentration of the substance and the intensity of the emission registered by the spectrofluorometer. Moreover, assuming that higher states initiate fluorescence, it should be noted that energy will be dissipated by vibrational relaxation and internal conversion. These new standards, fall between the two standards that framed the concentration of our tonic sample, to help us create a more accurate calibration curve. The excitation wavelength for riboflavin was 450 nm and the peak absorbance was also 1.

Next

Fluorescence Spectroscopy

fluorescence spectroscopy lab report quinine

A colorimeter is a type of… Murder Mystery Viking Experiment In order to complete the objective, the group used a Carolina Digital Spectrophotometer, unit M 201-1, which measures the quantity of light that solutions absorb. As a result, the maximum peak is noticeable at the longest incident wavelength. Theses shorter wavelengths involved X-rays and ultraviolet radiation. Concentration quenching results from excessive absorption of either the excitation or fluorescence radiation by the analytes in solution, which results in a decrease in the fluorescence-per-unit-concentration of analyte as the concentration is increased. Absorbance is the measure of light intensity that is absorbed by a sample. The reason for this effect lies in the simultaneous phenomena of fluorescence and internal transformations initiated by photon absorption.

Next

Quinine in Tonic Water with Fluorescence Spectroscopy

fluorescence spectroscopy lab report quinine

There are several types of quenching is inner filter or more commonly known as concentration quenching which refers to when the concentration is increased causes a decrease in the fluorescence-per —unit- concentration. L is the distance travelled by the radiation from the light source. The second solution that was prepared was KHP. During this process, electrons are excited from their standard state. In configuring the instrument, the CVD-diffuse cuvette was utilized to diffusely reflect the light to the detector which was configured to let light diffuse through the sample and reflect towards the detector. Compare and contrast fluorescence and chemiluminescence with respect to instrumentation requirements and design.

Next

Lab 7 fluorescence 2022

fluorescence spectroscopy lab report quinine

In the case of the internal effect, the heavy atom is chemically incorporated into the molecule under study and covalently bound to it. Quinine may cause a skin irritation or a possible skin allergy. A total of seven solutions with different dilutions were used throughout the lab to conduct the equilibrium constant. Learn More In fact, quinine has several peaks responsible for different transitions. Miguel Garcia Chemistry 221-801 April 17, 2017 Determination of Quinine in Tonic Water by Fluorescence Spectroscopy Objective : The purpose of the experiment was to determine the concentration of quinine in an unknown Tonic Water sample. In measuring the fluorescence intensity, the instrument was prepared to measure the Tonic water sample by considering the absorption of light at ~350 nanometers and emission of light at ~500 nanometers. A spectrophotometer was used in the lab to determine these values.

Next

Spectroscopy Laboratory Quinine in Tonic Water with...

fluorescence spectroscopy lab report quinine

That was seen as chlorine had the lower quenching which corresponded with the values seen. Aniline is an example. The addition of these solutions formed five test tubes of different dilutions, but of equal volume, 10 mL each. It also states that the absorption of light is… Chemistry 116 lab review The first step is to calibrate the colorimeter with0. Characterization and Determination of Quinine by Spectrofluorimetry Theory Molecular fluorescence is an important analytical method best characterized by the extremely low limits of detection possible for many molecules. The Chromophores are portions that contain electronic bands within a molecule which has an energy differences similar to the reading or energies seen from the UV-Vis light. It was important to do this right at the beginning of the lab since the zeroed value of the acid was the calibration number for all of the other solutions.

Next

[PDF] Fluorescence Determination of Quinine in Tonic Water Experiment

fluorescence spectroscopy lab report quinine

Why is fluorescence more sensitive than absorbance spectrophotometric methods? Once this was done, 0. At this wavelength, the peak absorbance was 1. For this purpose, a sample of the calibration solution was placed in the cell of the apparatus and measured at a photomultiplier tube voltage range of 400 V to 800 V in increments of 50 V. This statement was used for this laboratory work, in which the concentration of quinine in a sample of tonic water was quantified by measuring the fluorescent emission intensity of the substance. It fluoresces as the molecule between pH 5 and pH 13, below pH 5 it exists as the cation, and above pH 13 it exists as the anion; both do not fluoresce.

Next

Fluorescence Lab typemoon.org

fluorescence spectroscopy lab report quinine

Figure below is calibration graph of average fluorescence intensity Vs Quinine conc. Experimental Procedure: The materials required for this experiment were a 0. The fluorescent light then strikes a photomultiplier tube, which transforms the light signal into electrical energy. We as a group had four tubes labeled two, three, four, and five. Additional aims analysis an iron supplement of choice by the process of UV- Vis spectrophotometer, examine how chloride causes the quenching of fluorescence, bromide and iodide ions. The iron- phenanthroline complex intensely coloured and for analysis pH is not required to be kept under control but 3 are optimal. For part D, the final part of the experiment, the experiment is designed to show how measurements of quinine can demonstrate how by adding Cl,Br and Iodine it will reduce the intensity of the fluorescence.


Next

Experiment #3 Fluorescence Determination of Quinine

fluorescence spectroscopy lab report quinine

After selecting the actual empirical parameters, spectroscopic measurements were made for a series of previously prepared solutions. To make this easier to understand, think of a rock being picked up. To put it another way, a voltage of 700 V was the maximum sufficient to carry out further investigations. The linear regression for working standard solutions is shown in Figure 3. A dark current and reference signal was produced to zero the detector with regards to the atmospheric. And in the case of the electron, the energy that is released is light energy instead of sound energy. We first determined the intensity of our first five standards, then of our tonic sample, then of our four new standards.

Next